In my junior year, my classmates and I succeeded in obtaining funding from the National University Student Innovation Program with a project on “The Orthogenesis of Transketolase and Its Application in the Intermediate Synthesis of Chiral Drugs,” which intended to help patients suffering from liver metabolic disorders. This was the first time that I encountered the concept of orthogenesis, so I spent much time consulting relevant literature to understand it fully. The key to this research was to use the orthogenesis method to determine the mutation site of Transketolase rapidly, which impelled me to learn the protein structure, how to use the NCBI database to get protein structure information, and how to apply modeller and PyMOL to simulate the protein structure independently. We finally determined four possible mutation sites and realized we could design subsequent experiments to compare the effects of these four mutation sites and their combinations. Because my group partners and I emphasized the importance of collaboration, this achievement can be credited to our shared effort and communicative teamwork. What’s more, I learned how to manage my experimental plans and balance my time between studying and conducting my research, which I found immediately valuable.